Virus:Article Title: Junctional adhesion molecule C limits glioblastoma stem-like cell invasion by regulating integrin adhesion at the endothelial interface
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies JAMC Bethyl Cat# A303-761A; RRID:AB_11204755 JAMC-APC Biotechne Cat# FAB11891A; RRID:AB_2280616 JAMC R&D Cat# AF1189; RRID:AB_354658 Fibronectin Sigma Aldrich Cat# F3648; RRID:AB_476976 Collagen IV Novotec Cat# 20411-1; RRID: AB_3713204 Paxilin Millipore Cat# 05–417; RRID:AB_309724 Vimentin Santa Cruz Biotechnology Cat# sc-6260; RRID:AB_628437 Vimentin Abcam Cat# ab92547; RRID:AB_10562134 INTEGRINβ1 Santa Cruz Biotechnology Cat# sc-374429; RRID:AB_11012020 INTEGRINβ1-APC Invitrogen Cat# 17-0299-42; RRID:AB_1659684 INTEGRINβ3 Santa Cruz Biotechnology Cat# sc-365679; RRID:AB_10844835 INTEGRINβ5 Santa Cruz Biotechnology Cat# sc-398214; RRID: AB_3713205 INTEGRINβ8 Santa Cruz Biotechnology Cat# sc-514150; RRID:AB_3713206 SHARPIN Bethyl Cat# A303-559A; RRID:AB_10971166 HOIP Bethyl Cat# A303560A; RRID:AB_10949139 HOIL-1 Santa Cruz Biotechnology Cat# sc-393754; RRID:AB_371207 α-TUBULIN Proteintech Cat# 66031-1-Ig; RRID:AB_11042766 GAPDH Santa Cruz Biotechnology Cat# sc-32233; RRID:AB_627679 Goat Anti-Mouse Ig, Human ads-HRP Southern Biotech Cat# 1010-05; RRID:AB_2728714 Goat Anti-Mouse IgG1, Human ads-HRP Southern Biotech Cat# 1070-05; RRID:AB_2650509 Goat Anti-Mouse IgG2a, Human ads-HRP Southern Biotech Cat# 1080-05; RRID:AB_2734756 Goat Anti-Mouse IgG2b, Human ads-HRP Southern Biotech Cat# 1090-05; RRID: AB_2794521 Goat Anti-Rabbit IgG(H + L), Mouse/Human ads-HRP Southern Biotech Cat# 4050-05; RRID:AB_2795955 Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 Life Technologies Cat# A-21123; RRID:AB_2535765 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Life Technologies Cat# A-11035; RRID:AB_2534093 Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A-21121; RRID:AB_2735764 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Life Technologies Cat# A-11034; RRID:AB_2576217 Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Life Technologies Cat# C7373-03 Biological Samples Patient-derived glioblastoma stem-like cells GSC#1, GSC#2, GSC#6, GSC#9 Harford-Wright et al. 7 N/A GSC#1 WT This paper N/A GSC#1 JAMC − /− This paper N/A GSC#9 WT This paper N/A GSC#9 JAMC − /− This paper N/A GSC#9 sgCtrl This paper N/A GSC#9 GFP André -Gré goire et al. 61 N/A (Continued on next page) 18 Cell Reports 44, 116194, September 23, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER GSC#9 WT GFP empty vector This paper N/A GSC#9 WT GFP SHARPIN This paper N/A GSC#9 JAMC − /− GFP empty vector This paper N/A GSC#9 JAMC − /− GFP SHARPIN This paper N/A GSC#9 sgCrtl True Guide This paper N/A GSC#9 sgJAMC True Guide This paper N/A Chemicals, peptides, and recombinant proteins Isolectin GS-IB4 Life Technologies Cat# I21413 Green Cell Tracker CMFDA Invitrogen Cat# C2925 Red Cell Tracker CMTPX Invitrogen Cat# C34552 Ponceau S Solution Santa Cruz Biotechnology Cat# sc-301558; CAS: 6226-79-5 Paraformaldehyde (PFA) Electron Microscopy Sciences Cat#15710; CAS: 30525-89-4 Triton X-100 Merck Millipore Cat# T9284; CAS: 9036-19-5 Bovine Serum Albumin (BSA) Merck Millipore Cat# A2153; CAS: 9048-46-8 DAPI Solution Life Technologies Cat# 62248; CAS: 28718-90-3 Glycin Eurobio Scientific Cat# GEPGLY00-66; CAS: 56-40-6 Matrigel Corning Cat# 356237 Collagen I Corning Cat# 354236 Puromycin Sigma-Aldrich Cat# P7255; CAS: 58-58-2 Amonium hydroxylate Sigma-Aldrich Cat# 221228; CAS: 1113-38-8 Methylcellulose Sigma-Aldrich Cat# M0512; CAS: 9004-67-5 Sucrose Sigma-Aldrich Cat# S0389; CAS: 57-50-1 Puromycin dihydrochloride ThermoFisher Cat#P8833; CAS: 58-58-2 Critical commercial assays BC assay: protein assay kit Interchim Cat# FT-40840A CellTiter-Glo 2.0 Cell Viability Assay Promega Cat# G9243 Deposited Data Label-free quantification proteomic analysis of total cell lysates from GSC#1 WT versus GSC#1 JAMC − /− PRIDE PXD062679 3 ′ SRP WT versus JAMC − /− GSC GEO GSE302919 Gene expression profiling of human glioblastoma cells under culture with endothelial cell condition media using Affymetrix arrays GEO GSE302671 RNA-seq analysis of spatial distribution of GBM DRYAD, Ravi et al., 35 h70rxwdmj Experimental models:Cell lines hCMEC/D3 Weksler et al. 61 Gift from Couraud P.O. HEK-293T ATCC Cat# CRL-3216; RRID:CVCL_0063 CT-2A Merck Cat# SCC194; RRID: CVCL_ZJ44 Experimental models: Organisms/strains Mouse: BALB/CAnN.Cg-Foxn1 nu/nu Charles River N/A Mouse: C57BL/6 mice Charles River N/A Oligonucleotides Non-silencing low-GC RNA duplexes Life Technologies Cat# 12935111 siRNAs targeting human JAM3 Life Technologies Cat# HSS130323 Mixture of siRNAs ON-TARGETplus human PARD3 Dharmacon Cat# L-015602-00-0005 (Continued on next page) Cell Reports 44, 116194, September 23, 2025 19
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Bethyl is a verified supplier 